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Our lab is characterizing the S. solfataricus proteome by estabishing the protein composition of wild type and targeted disruption mutant strains. Two dimensional SDS PAGE of total protein extracts separates proteins based on mass and charge. Selected proteins are Trypsin hydrolyzed and the resulting peptides fractionated by capillary electrophoresis. Appropriate peptides are identified by tandem mass spectrometry (MS/MS) followed by matching to the S. solfataricus sequenced genome.  |